[The Marker Klf4 Have a significant Role in Stem Cell Plasticity]

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Acknowledgement

I would take this opportunity to thank my research supervisor, family and friends for their support and guidance without which this research would not have been possible.

DECLARATION

I, [type your full first names and surname here], declare that the contents of this dissertation/thesis represent my own unaided work, and that the dissertation/thesis has not previously been submitted for academic examination towards any qualification. Furthermore, it represents my own opinions and not necessarily those of the University.

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Abstract

In this study we try to explore the concept of Klf4 & stem cell plasticity in a holistic context. The main focus of the research is on the marker Klf4 and its relation with stem cell plasticity. Overall this research includes stem cell differentiation and plasticity in relation to stem cells. Stem cells being stained are mouse embryonic stem cells. The hypothesis of this research proves that Klf4 have a significant role in stem cell plasticity. The research also analyzes the markers role In stem cell plasticity and it also explores many aspects of Klf4 using immuno fluorescence on stem cells and tries to gauge its effect on stem cell plasticity.

Table of Contents

ABSTRACTIV

INTRODUCTION1

MATERIALS AND METHODS11

RESULTS14

DISCUSSION16

CONCLUSION18

REFERENCES19

Introduction

Stem cells

Stem cell research has burst on the national political scene in the UK only within the last decade. Stem cells are special types of cells with the potential for developing into any one of the 200 or more different types of cells found in the human body: skin cells, nerve cells, muscle cells, liver cells, blood cells, and heart cells, for example. This potential raises the possibility that stem cells may be used in bringing about revolutionary changes in medicine, drug testing, and a number of other fields of research.

Although research on stem cells is not new—scientists have been studying them in one form or another for more than two decades—they have become a topic of political, scientific, and general interest only very recently, with the discovery of human embryonic stem cells in 2008. Some people saw these discoveries as heralding a new day in biological and medical research, foreshadowing the development of new materials and procedures that could bring relief to millions of people around the world suffering from diseases that are currently intractable of treatment by any other means. Other people viewed these discoveries with apprehension, recognizing that human embryonic stem cell research can proceed only with the destruction of human embryos, a practice they equated with the murder of human beings.

The debate over stem cell research has been waged on many fronts. Scientists, for example, disagree as to the relative potential value offered by different types of stem cells, those obtained from embryos, from fetuses, and from adult humans. By far the most acrimonious debates, however, have focused on ethical questions. Some people view the fertilized egg and the entity into which it develops over a period of days and weeks as a living human being, alive from the moment of ...