Abstract

Bacteria are considered as tiny organisms, which range from a length of one micrometer to ten micrometers. Pathogen detection and bacterial detection is necessary for the correct identification and diagnosis of disease. Microbiologists observe the characteristics of colonies on generic agar that provides a wide variety of nutrients. In hospitals, the correct and rapid identification of a bacterial pathogen may save someone's life. Sterilization is a process essential to the operation of a hospital, which must use all the surgical instruments, implants and many other devices sterile.

Microarrays provide the identification through the simultaneous bacteria identification and species identification

Development Of Microbial Identification

Introduction

Bacteria are considered as tiny organisms, which range from a length of one to ten micrometers. The largest specie of these bacteria is known as Thiomargarita namibiensis with a diameter of 100 to 750 micrometers. In 1990, the researchers identified these microorganisms and they differentiated them to nano-bacteria. It is necessary that the accurate and definitive microorganism is necessary for further medical research. Pathogen detection and bacterial detection is also necessary for the correct identification and diagnosis of disease. The treatment of infection is only possible by the treatment of infection and keeping a track of the disease, which are associated with microbial infections. Bacterial identification is used for a variety of applications, which include criminal investigation, microbial forensics, environmental studies and bio terrorism threats.

Bacteria Identification

Among all, different types of microorganisms, bacterial identification make the broadest use of physical, biochemical, and genetic methods. Physical differentiation of bacteria begins with the morphology of bacterial colonies on general growth and differential growth (Siegetsleitner, pp. 1036). Colonies of a given genus look the same if grown on the same type of agar. This is true whether two different cultures of a species incubate a day apart or a year apart. Microbiologists observe the characteristics of colonies on generic agar that provides a wide variety of nutrients (Watson and Crick, pp. 737). On this type of agar, species produce a variety of colony types such as smooth versus rough, small versus large, dome-shaped versus flat, white versus gray. Microbiologists, supplement this finding by growing the bacteria on differential agar media, which do a better job at distinguishing between major groups of bacteria. For example, eosin methylene blue (EMB) agar is a differential medium containing the dyes eosin and methylene blue (Yount, pp. 98).

Microbiologists have developed several hundred different types of media to separate species by morphology and biochemistry at the same time. The table describes some of the common identification techniques used by most laboratories for bacteria. In hospitals, the correct and rapid identification of a bacterial pathogen may save someone's life (Watson and Crick, pp. 737). Many of the methods are accurate but require one or more days to complete. Since the early 1990s, biological engineers have invented instruments that provide "rapid identification." Three main types of rapid systems are (1) manual biochemical kits, (2) fatty acid analysis, and (3) serology. These systems are called rapid because an unknown bacterium can be identified in less than an ...