Urinary Tract Infection Based On The Gram Staining And Cled Colony Identification

Abstract

To evaluate the frequency of the bacterial agents responsible for urinary tract infections and the antimicrobial sensitivity profile of the uropathogens involved in these infectious processes. Methods - A survey was conducted of the data with reference to uroculture exams of patients attended at the Military Police Hospital of the State of Goias, Brazil, in the period from January, 1998 to December, 2008, in which it was verified that of the 10,162 urine samples, 1,506 (14.82%) were positives, while 8,656 (85.18%) presented no growth of microorganisms. Results - According to the results, it was verified that the female sex was the most affected (79.4%). The most frequent Gramnegative bacteria were Escherichia coli (63.08%) and Enterobacter sp. (6.31%) and the Gram-positive bacteria were Staphylococcus saprophyticus (4.52%) and Staphylococcus aureus (3.19%). Conclusion - With regard to bacterial susceptibility to antimicrobial agents, it was noted that Escherichia coli and Enterobacter sp. presented a higher sensitivity index in decreasing order to Ciprofloxacin, Norfloxacin and Ceftriaxon.

Urinary Tract Infection Based On The Gram Staining And Cled Colony Identification

Introduction

Urinary tract infection (UTI) consists of microbial invasion and multiplication in any of the structures of the urinary system. The severity of infection ranges from asymptomatic colonization, or that is, without tissue degeneration through to symptomatic invasion of the tissues of any of the structures of the urinary system1-2 . World-wide, a minimum occurrence of 150 million symptomatic cases of UTIs are observed every year, however, the real incidence is underestimated because a large portion of urinary infections are cured without medical follow-up 3 . The etiology of UT infections is related to the great diversity of microbial invaders, such as: bacteria, viruses and fungi. The etiologic agents most commonly isolated in UTIs are: Enterobacteriaceae family of gram-negative bacilli, its main representative being Escherichia coli, with a mean frequency of 40% to 85% among the nosocomial and ambulatorial cases, respectively 4 . Others genuses of this family are involved, however, having a lower incidence, such as: Enterobacter sp., Citrobacter sp., Proteus sp., Klebsiella SP 1,3,5-7 . In the United States, the non complicated UT infections constitute the main cause of morbidity among women, which results in around eight million consultations per annum. This is no different in Brazil. In ambulatorial clinical practice, UTIs are responsible for 80% of the medical attendances, and they are considered one of the most common bacterial affections, particularly in children, sexually active young adults and women, ranked lower in frequency only to respiratory infections 2,8 . The diagnosis of UTI is based on clinical and laboratorial findings. The finding of bacteria in the urine is a strong indication of urinary infection, mainly when the collection, transport and storage of the urine sample met the criteria recommended for these procedures 1,9 . Undoubtedly, the method considered the gold standard for the diagnosis of UTI is uroculture, as it allows the colony forming units per mL of urine (CFU/mL) to be counted, and ...