Measurements of Plasma Glucose, Cholesterol and Protein

Introduction

The connection between diet and chronic infection risk is complex and multi-factorial. There are a number of biological components that are altered by diet. In turn, some of these factors may individually or in blend alter cardiovascular infection (CVD) risk. Data on the connection between both saturated and trans fatty unpleasant intake, and plasma lipid levels are somewhat consistent. When either saturated and trans fatty acids are exchanged for cis unsaturated fatty acids, total and LDL cholesterol grades are higher. When trans fatty acids are exchanged for saturated fatty acids, HDL cholesterol grades are lower, triglyceride grades are higher and the total cholesterol to HDL cholesterol ratio is less favorable.

The outcomes of some large-scale epidemiological investigations propose that trans fatty acid intake is associated with an increased risk of developing CVD, possibly larger than would be predicted from changes in plasma lipid levels alone. It has further been described that trans fatty acid intake is associated with an advanced risk of evolving kind 2 diabetes. In compare, facts and figures from lesser studies suggested that trans fatty unpleasant intake is not affiliated with markers of insulin resistance. Saturated fat intake is positively associated with bigger LDL cholesterol grades and CVD risk and has been affiliated with a deterioration of glucose control.

Methods

Thirty-six female (n=18) and male (n=18) subjects (age range females, 57-73 years; males, 52-73 years) with LDL cholesterol levels >130 mg/dl were recruited for this study from the greater Boston area. Twenty-three subjects took part in the postprandial studies. All topics fulfilled the following criteria: usual kidney, liver, thyroid and cardiac function; normal fasting glucose grades; not taking medications renowned to sway body-fluid lipid levels; and non-smoker. All females were postmenopausal and not taking hormone replacement therapy. Subjects treated for hypertension were excluded only if they were using ß-blocker therapy. Subjects were counseled not to change habitual grades of physical activity and medication use throughout the study period.

Sample Results

The data was collected in a previous physiology course and reflects the same procedures we use in our experiments. For the plasma glucose and cholesterol concentrations, the concentration of the unknown blood sample, the Normal Serum sample and the abNormal Serum sample using Beer's law were calculated. For plasma protein concentrations, the concentration of each value by two methods were calculated. Beer's law and a standard curve from a plot of the five standard values were calculated.

Analysis

Low blood glucose (hypoglycemia) can cause fatigue, but there are other more common causes of fatigue for people with HIV. In a healthy person, blood glucose is controlled by insulin. Insulin is a hormone produced by the pancreas and helps glucose move from your blood into your cells to produce energy. High blood glucose levels could mean that your pancreas is not making enough insulin. However, some persons make abounding of insulin, but their body doesn't respond normally. This is called “insulin resistance.” In either case, the cells don't get sufficient glucose to use for power, and glucose builds up in ...