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Phlebotomy is one of the most important steps in the work of the laboratory clinician. On the one hand represents the first contact between the laboratory and their patients and from the point of view of the sample blood, the enormous importance of dealing with a sample collected properly, the safety of their origin and proper packaging and transport , are key factors the assessment and report of tests performed. The staff has to make the blood sample collection, keep in mind that the proper treatment of the patient, orientation and ability to do its work , is the face of the Clinical Laboratory to the community to be served. This protocol is intended as a guide to staff responsible for the collection of blood samples, such that this function is standardized in its technical aspects and establishing the necessary guidelines on bio-safety and factors that may affect the quality of the sample (Lippi and Banfi, 2007).

Safety First

General requirements for personal safety

Clothing & Accessories: It is mandatory to use beat throughout the time working in the laboratory. When it comes to extracting sample is required to use protective gloves. It is advisable to use protective eyewear.

No eating or drinking in work area.

Smoking is not allowed.

Specific measures for phlebotomy

Minimum Practice universal precautions with every patient to be treated.

Each sample should be considered potentially infectious and should take precautions to ensure the safety of the phlebotomist and patient.

Clean your table before starting work.

If the patient coughs frequently, wear a mask.

Avoid touching the patient visibly infected areas.

Take precautions when handling needles and lancets.

Do not leave needles and lancets in the desk.

Immediately discard the container.

Do not place the needle guard.

If there is spilled blood , wipe with sodium hypochlorite 10%.

Clean tourniquet alcohol ethyl after each extraction.

Change gloves if they are stained with blood or other bodily fluids.

Whenever possible, change gloves between patients.

After work, remove gloves and wash hands thoroughly.

If there is a needle prick a patient's blood containing HIV positive, immediately inform the head of the lab and go to the clinic employee or risk professionals. Follow the instructions on the basis of the Protocol accidents occupational institution.

Do not allow children to handle or play with teams of phlebotomy.

Syringe extraction

When you proceed with the extraction syringe, you should keep in mind the size used and the size of the syringe with the volume to be extracted (Dunn and Moga, 2010).

a) Place the tip of the needle at an angle of 15 to 30 degrees on the surface of the chosen vein and through the skin with a moving firm and sure, until the lumen of the vein.

b) Tighten the syringe plunger should be pulled continuous movement to draw blood to the required volume. Avoid slamming on the needle during removal.

c) Loosen tourniquet for blood to flow well and remove the needle with smooth movement of the arm at the end of ...
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