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substrate with varying levels of each, as well as varying time allotted to reaction. Enzymes are catalytic proteins, meaning they speed up chemical reactions without being used up or altered permanently in the process. Although various enzy...
RNA from Arabidopsis tissues. As far as we know, all non-commercial protocols for RNA isolation from plant tissues use organic solvents during the first steps. We present here a protocol that uses two simple buffers without organic solvents...
DateTask 1 Definition The Bradford protein assay is one of several simple methods commonly used to determine the total protein concentration of a sample. The method is based on the proportional binding of the dye Coomassie to proteins. With...
evidence-based instruction is the integration of professional wisdom with best available empirical evidence (Whitehurst, 2002). Basically, evidence-based instruction is the methodology, program and/or practice having records of success. Val...
hydration of an alkene in an aqueous acid, which is considered to be an important industrial process (Bettelheim, 2013, pp. 82). This experiment in turn also involves the treatment of 1-hexene with dilute sulphuric acid first at room temper...
lung protective strategy is predicated exclusively on the application of a specific low tidal volume (Vt) is incomplete. In the most widely quoted Acute Respiratory Distress Syndrome Network (ARDS-net) study, it is true that Vts were limite...
list of exotic fish diseases in European Union (EU) legislation. The criteria for listing a disease as exotic are that disease i) is not established in Community and pathogen is not known to be present and ii) it has potential for either s...